Ask AI: Describe step by step what occurs in adenine base editing

1. First, the CRISPR-associated protein (Cas) binds to the single-guide RNA (sgRNA) and adenine base editor (ABE) complex. This enables the complex to locate the target DNA sequence within the genome. 2. The ABE complex then binds to the target sequence, forming a specific base pair. 3. The CRISPR-associated protein (Cas) then activates adenine deaminase, which catalyzes the conversion of the adenine (A) in the target DNA sequence to inosine (I). 4. Inosine is then recognized as guanine (G) by the cellular machinery and then incorporated into the DNA sequence. 5. Finally, the new DNA sequence is expressed by the cell, leading to an altered gene sequence, which can have a wide range of effects on the phenotype of the organism.