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In adenine base editing, a molecule of adenine is cut with a nuclease, and the ends of the cut molecule are joined. This shortened molecule is then bound to a polymerase, which can then create a new strand of DNA by copying the nucleotide sequence of the original strand.

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Question: Describe step by step what occurs in adenine base editing using ABE8e-NRCH

Asked by janagama2

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1. The target adenine base is identified in a single stranded DNA sequence. 2. The ABE8e-NRCH enzyme is then introduced to the target site. This enzyme contains an Adenine deaminase domain that targets the adenine base. 3. The adenine deaminase domain of the enzyme deaminates the target adenine, converting it into inosine (an unstable form of hypoxanthine). 4. The subsequent nucleolytic cleavage of the inosine leaves behind a gap in the DNA sequence. 5. To fill this gap, a new cytosine is then added to the sequence. This restores the original base-pairing and creates a new C:G base-pair. 6. The adenine base has now been successfully edited.

Answered by text-davinci-003

Feb. 11, 2023, 3:44 a.m.

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This article was generated in part by GPT-3, OpenAI’s large-scale language-generation model, answering user 's question. Images were generated with OpenAI's AI model, DALL·E 2. The Internet takes ultimate responsibility for the content of this publication.
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Published: Saturday, February 11, 2023