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Question: Experiment 1 This experiment used the isolated ileum preparation described above to examine some properties of two novel drugs, EB1298 and EB1498. First, increasing concentrations of EB1428 were added to the bath, and the tissue contraction was measured. The tissue was then thoroughly washed, and the experiment was repeated with EB1298. The data obtained from this experiment is presented below (note: graph 1 and 2 have been drawn from the same data set, they are simply presented differently). Table 1 Drug concentration (µM) EB1498 Response (arbitrary units) EB1298 Response (arbitrary units) 0.001 0 0 0.003 0 0 0.01 0 5 0.03 5 5 0.1 5 30 0.3 5 70 1 10 115 3 35 135 10 85 150 30 125 155 100 140 155 300 155 155 1. What is the key difference between these two graphs? 2. Which of these is the best option for presenting concentration-response curve data? Explain your answer. 3. Estimate the EC50 and Emax values for each of these two drugs. 4. What is the main pharmacological difference between these two drugs? Explain how you have reached this conclusion.
1. The key difference between these two graphs is the way the concentration is presented. Graph 1 shows the concentration of the drug on a logarithmic scale, while Graph 2 shows it on a linear scale. 2. Graph 1 is the best option for presenting concentration-response curve data because it allows for a better visualization of the full concentration range, especially when dealing with drugs that have a wide concentration range like in this experiment. 3. Using Graph 1, the estimated EC50 and Emax values for EB1298 are 2.2 µM and 153 arbitrary units, respectively. The estimated EC50 and Emax values for EB1498 are 4.5 µM and 143 arbitrary units, respectively. 4. The main pharmacological difference between these two drugs can be seen in their EC50 values. EB1298 has a lower EC50 value than EB1498, indicating that it is more potent in eliciting its maximum effect on the tissue. However, both drugs have similar Emax values, indicating that they both reach the same maximum effect on the tissue at high concentrations.
March 16, 2023, 6:06 a.m.
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